Main Information

Target

Cyclophilin B

Host Species

Mouse

Reactivity

Human, Rat, Mouse

Applications

IF, WB, IHC,

Conjugate/Modification

Biotin

Detailed Information

Recommended Dilution Ratio

Optimal working dilutions should be determined experimentally by the investigator; Suggested starting dilutions are as follows:WB 1:1000-2000; IHC 1:50-300.

Formulation

Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.

Specificity

Cyclophilin B Monoclonal Antibody(2B10) Biotin conjugated specially designed for your WB or IHC analysis.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Concentration

1mg/ml

Modification

Unmodified

Conjugate

Biotin

Clonality

Monoclonal

Clone Number

2B10

Isotype

IgG

Antigen&Target Information

Specificity:

Cyclophilin B Monoclonal Antibody(2B10) Biotin conjugated specially designed for your WB or IHC analysis.

Gene Name:

PPIB

Protein Name:

Peptidyl-prolyl cis-trans isomerase B (PPIase B) (CYP-S1) (Cyclophilin B) (Rotamase B) (S-cyclophilin) (SCYLP)

Other Name:

Cyclophilin B

Database Link:

Organism	Gene ID	SwissProt
Human	5479;	P23284;

Background:

The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009],

Function:

Catalytic activity:Peptidylproline (omega=180) = peptidylproline (omega=0).,Caution:It is uncertain whether Met-1 or Met-9 is the initiator.,enzyme regulation:Cyclosporin A (CsA) inhibits CYPB.,Function:PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.,similarity:Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.,similarity:Contains 1 PPIase cyclophilin-type domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,

Cellular Localization:

Virion . (Microbial infection).; Endoplasmic reticulum lumen . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). .

Tissue Expression:

Brain,Fetal brain cortex,Prostate,Skin,