Main Information

Target

Cyclophilin B

Host Species

Mouse

Reactivity

Human, Rat, Mouse

Applications

IF, WB, IHC,

Conjugate/Modification

Cy3

Detailed Information

Recommended Dilution Ratio

Optimal working dilutions should be determined experimentally by the investigator; Suggested starting dilutions are as follows:IHC 1:50-300; IF 1:50-200.

Formulation

Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.

Specificity

Cyclophilin B Monoclonal Antibody(2B10) Cy3 Conjugated specially designed for your Immunofluorescence analysis.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.

Concentration

1mg/ml

Modification

Unmodified

Conjugate

Cy3

Clonality

Monoclonal

Clone Number

2B10

Isotype

IgG

Antigen&Target Information

Specificity:

Cyclophilin B Monoclonal Antibody(2B10) Cy3 Conjugated specially designed for your Immunofluorescence analysis.

Gene Name:

PPIB

Protein Name:

Peptidyl-prolyl cis-trans isomerase B (PPIase B) (CYP-S1) (Cyclophilin B) (Rotamase B) (S-cyclophilin) (SCYLP)

Other Name:

Cyclophilin B

Database Link:

Organism	Gene ID	SwissProt
Human	5479;	P23284;

Background:

The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009],

Function:

Catalytic activity:Peptidylproline (omega=180) = peptidylproline (omega=0).,Caution:It is uncertain whether Met-1 or Met-9 is the initiator.,enzyme regulation:Cyclosporin A (CsA) inhibits CYPB.,Function:PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.,similarity:Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.,similarity:Contains 1 PPIase cyclophilin-type domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,

Cellular Localization:

Virion . (Microbial infection).; Endoplasmic reticulum lumen . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). .

Tissue Expression:

Brain,Fetal brain cortex,Prostate,Skin,