pGL4.11[luc2P] 载体
质粒类型: 启动子报告载体
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4370 bp
出品公司: promega
载体抗性: Ampicillin (氨苄青霉素)
备注: Promoterless vector encoding destabilized luciferase for measuring the activity of promoter and enhancer sequences.
载体描述
The pGL4.11[luc2P] Vector(a–d)encodes the luciferase reporter gene luc2P (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.The pGL4.11[luc2P] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.