Main Information

Target

Adrenocorticotropin

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF, WB

MW

29kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human Adrenocorticotropin(ACTH)

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

29kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human Adrenocorticotropin(ACTH)

Specificity:

This antibody detects endogenous levels of human Adrenocorticotropin(ACTH)

Gene Name:

POMC

Protein Name:

Adrenocorticotropin(ACTH)

Other Name:

Pro-opiomelanocortin ;
POMC ;
Corticotropin-lipotropin ;
[Cleaved into: NPP ;
Melanotropin gamma ;
Gamma-MSH ;
Potential peptide ;
Corticotropin ;
Adrenocorticotropic hormone ;
ACTH ;
Melanotropin alpha ;
Alpha-MSH ;
Corticotropin-like intermediary peptide ;
CLIP ;
Lipotropin beta ;
Beta-LPH ;
Lipotropin gamma ;
Gamma-LPH ;
Melanotropin beta ;
Beta-MSH ;
Beta-endorphin ;
Met-enkephalin]

Database Link:

Organism	Gene ID	SwissProt
Human	5443;	P01189;

Background:

This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. The

Function:

Disease:Defects in POMC are the cause of pro-opiomelanocortinin deficiency [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair.,Disease:Defects in POMC may be associated with susceptibility to obesity [MIM:601665].,Function:ACTH stimulates the adrenal glands to release cortisol.,Function:Beta-endorphin and Met-enkephalin are endogenous opiates.,Function:MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.,online information:Melanocyte-stimulating hormone entry,PTM:O-glycosylated; reducing sugar is probably N-acetylgalactosamine.,PTM:Specific enzymatic cleavages at paired basic residues yield the different active peptides.,similarity:Belongs to the POMC family.,tissue specificity:ACTH and MSH are produced by the pituitary gland.,

Cellular Localization:

Secreted . Melanocyte-stimulating hormone alpha and beta-endorphin are stored in separate granules in hypothalamic POMC neurons, suggesting that secretion may be under the control of different regulatory mechanisms. .

Tissue Expression:

ACTH and MSH are produced by the pituitary gland.

Research Areas:

>>cAMP signaling pathway ;
>>Neuroactive ligand-receptor interaction ;
>>Estrogen signaling pathway ;
>>Melanogenesis ;
>>Adipocytokine signaling pathway ;
>>Aldosterone synthesis and secretion ;
>>Cortisol synthesis and secretion ;
>>Cushing syndrome