Main Information

Target

COX1

Host Species

Rabbit

Reactivity

Human

Applications

WB

MW

70kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous COX1 proteins.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

70kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthetic Peptide of COX1 AA range: 483-533

Specificity:

The antibody detects endogenous COX1 proteins.

Gene Name:

PTGS1

Protein Name:

Prostaglandin G/H synthase 1

Other Name:

PTGS1 ;
COX1 ;
Prostaglandin G/H synthase 1 ;
Cyclooxygenase-1 ;
COX-1 ;
Prostaglandin H2 synthase 1 ;
PGH synthase 1 ;
PGHS-1 ;
PHS 1 ;
Prostaglandin-endoperoxide synthase 1

Database Link:

Organism	Gene ID	SwissProt
Human	5742;	P23219;
Mouse	19224;	P22437;
Rat	24693;	Q63921;

Background:

This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],

Function:

Catalytic activity:Arachidonate + AH(2) + 2 O(2) = prostaglandin H(2) + A + H(2)O.,cofactor:Binds 1 heme B (iron-protoporphyrin IX) group per subunit.,Function:May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.,miscellaneous:This enzyme acts both as a dioxygenase and as a peroxidase.,miscellaneous:This enzyme is the target of nonsteroidal anti-inflammatory drugs such as aspirin.,pathway:Lipid metabolism; prostaglandin biosynthesis.,similarity:Belongs to the prostaglandin G/H synthase family.,similarity:Contains 1 EGF-like domain.,subunit:Homodimer.,

Cellular Localization:

Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Tissue Expression:

Brain,Lung fibroblast,Platelet,

Research Areas:

>>Arachidonic acid metabolism ;
>>Metabolic pathways ;
>>Platelet activation ;
>>Serotonergic synapse ;
>>Regulation of lipolysis in adipocytes