Main Information

Target

HSPB8

Host Species

Rabbit

Reactivity

Human

Applications

WB

MW

22kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous Crystallin-αC protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

22kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of Crystallin-αC

Specificity:

The antibody detects endogenous Crystallin-αC protein.

Gene Name:

HSPB8

Protein Name:

Heat shock protein beta-8

Other Name:

HSPB8 ;
CRYAC ;
E2IG1 ;
HSP22 ;
PP1629 ;
Heat shock protein beta-8 ;
HspB8 ;
Alpha-crystallin C chain ;
E2-induced gene 1 protein ;
Protein kinase H11 ;
Small stress protein-like protein HSP22

Database Link:

Organism	Gene ID	SwissProt
Human	26353;	Q9UJY1;
Mouse		Q9JK92;

Background:

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008],

Function:

Caution:Was reported (PubMed:10833516) to have a protein kinase activity and to act as a Mn(2+)-dependent serine-threonine-specific protein kinase.,Disease:Defects in HSPB8 are the cause of Charcot-Marie-Tooth disease type 2L (CMT2L) [MIM:608673]. CMT2L is an axonal form of Charcot-Marie-Tooth disease. Axonal CMT neuropathies are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.,Disease:Defects in HSPB8 are the cause of distal hereditary motor neuronopathy type 2A (HMN2A) [MIM:158590]; also known as distal hereditary motor neuropathy type IIA or spinal Charcot-Marie-Tooth disease IIA. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.,Function:Displays temperature-dependent chaperone activity.,induction:By 17-beta-estradiol.,PTM:Phosphorylated.,similarity:Belongs to the small heat shock protein (HSP20) family.,subunit:Monomer. Interacts with HSPB1.,tissue specificity:Predominantly expressed in skeletal muscle and heart.,

Cellular Localization:

Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.

Tissue Expression:

Predominantly expressed in skeletal muscle and heart.