Main Information

Target

ACINU

Host Species

Rabbit

Reactivity

Human, Mouse

Applications

WB, ELISA

MW

147kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000; ELISA 1:5000-20000

Formulation

Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Specificity

ACINU Polyclonal Antibody detects endogenous levels of protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

147kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human protein . at AA range: 1250-1330

Specificity:

ACINU Polyclonal Antibody detects endogenous levels of protein.

Gene Name:

ACIN1 ACINUS KIAA0670

Protein Name:

Apoptotic chromatin condensation inducer in the nucleus (Acinus)

Database Link:

Organism	Gene ID	SwissProt
Human	22985;	Q9UKV3;
Mouse		Q9JIX8;

Background:

Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. This gene encodes a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. It may thus be involved in mRNA metabolism associated with splicing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011],

Function:

Function:Induces apoptotic chromatin condensation after activation by CASP3.,PTM:Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.,similarity:Contains 1 SAP domain.,subunit:Interacts with API5.,tissue specificity:Ubiquitous.,

Cellular Localization:

Nucleus. Nucleus speckle. Nucleus, nucleoplasm. Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.

Tissue Expression:

Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

Research Areas:

>>Nucleocytoplasmic transport ;
>>mRNA surveillance pathway ;
>>Spliceosome