Main Information

Target

AAT1

Host Species

Rabbit

Reactivity

Human, Mouse

Applications

WB, ELISA

MW

66kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000; ELISA 1:5000-20000

Formulation

Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Specificity

AAT1 Polyclonal Antibody detects endogenous levels of protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

66kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human protein . at AA range: 310-390

Specificity:

AAT1 Polyclonal Antibody detects endogenous levels of protein.

Gene Name:

AAT1 C3orf15

Protein Name:

AMY-1-associating protein expressed in testis 1 (AAT-1)

Database Link:

Organism	Gene ID	SwissProt
Human	89876;	Q7Z4T9;
Mouse		Q8BRC6;

Background:

alternative products:Additional isoforms (AAT-1L and AAT-1S) may exist,function:Isoform 4 may play a role in spermatogenesis.,PTM:Isoform 4 is phosphorylated by PKA.,subcellular location:Isoform 4 is found in the mitochondria and localized in the neck of the sperm.,subunit:Interacts with AMY1 and AKAP1. Isoform 4 belongs to a complex together with AMY1, AKAP1 and PRKAR2B. Isoform 3 does not interact with AMY1.,tissue specificity:Isoform 1 is strongly expressed in the liver. Isoform 4 is widely expressed, but strongly expressed in all spermatogenesis-related tissues, including the testis, the epithelium of cauda and the corpus epididymis. Isoform 4 is present in the spermatid and mature sperm. Isoform 4 is also expressed in Leydig cells. Isoform 3 is expressed in the testis.,

Function:

Alternative products:Additional isoforms (AAT-1L and AAT-1S) may exist,Function:Isoform 4 may play a role in spermatogenesis.,PTM:Isoform 4 is phosphorylated by PKA.,subcellular location:Isoform 4 is found in the mitochondria and localized in the neck of the sperm.,subunit:Interacts with AMY1 and AKAP1. Isoform 4 belongs to a complex together with AMY1, AKAP1 and PRKAR2B. Isoform 3 does not interact with AMY1.,tissue specificity:Isoform 1 is strongly expressed in the liver. Isoform 4 is widely expressed, but strongly expressed in all spermatogenesis-related tissues, including the testis, the epithelium of cauda and the corpus epididymis. Isoform 4 is present in the spermatid and mature sperm. Isoform 4 is also expressed in Leydig cells. Isoform 3 is expressed in the testis.,

Cellular Localization:

Mitochondrion . Cytoplasm . Cytoplasm, cytoskeleton, cilium axoneme . Localized in the neck of the sperm.; [Isoform 7]: Cytoplasm .

Tissue Expression:

[Isoform 1]: Strongly expressed in the liver.; [Isoform 7]: Widely expressed, but strongly expressed in all spermatogenesis-related tissues, including the testis, the epithelium of cauda and the corpus epididymis, as well as the spermatid and mature sperm. Also expressed in Leydig cells.