Main Information

Target

p70 S6 kinase α

Host Species

Rabbit

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF, ELISA

MW

60kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

p70 S6 kinase α Polyclonal Antibody detects endogenous levels of p70 S6 kinase α protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

60kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase. AA range:337-386

Specificity:

p70 S6 kinase α Polyclonal Antibody detects endogenous levels of p70 S6 kinase α protein.

Gene Name:

RPS6KB1

Protein Name:

Ribosomal protein S6 kinase beta-1

Other Name:

RPS6KB1 ;
STK14A ;
Ribosomal protein S6 kinase beta-1 ;
S6K-beta-1 ;
S6K1 ;
70 kDa ribosomal protein S6 kinase 1 ;
P70S6K1 ;
p70-S6K 1 ;
Ribosomal protein S6 kinase I ;
Serine/threonine-protein kinase 14A ;
p70 ribosomal S6 kinase alpha ;
p70 S6 kinas

Database Link:

Organism	Gene ID	SwissProt
Human	6198;	P23443;
Mouse	72508;	Q8BSK8;
Rat	83840;	P67999;

Background:

ribosomal protein S6 kinase B1(RPS6KB1) Homo sapiens This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013],

Function:

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activation by serine/threonine phosphorylation and protein kinase C, inactivated by type 2A phosphatase.,Function:Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PPP1R9A/neurabin-1.,tissue specificity:Widely expressed.,

Cellular Localization:

Cell junction, synapse, synaptosome . Mitochondrion outer membrane. Mitochondrion. Colocalizes with URI1 at mitochondrion.; [Isoform Alpha I]: Nucleus. Cytoplasm.; [Isoform Alpha II]: Cytoplasm.

Tissue Expression:

Widely expressed.

Research Areas:

>>EGFR tyrosine kinase inhibitor resistance ;
>>Endocrine resistance ;
>>ErbB signaling pathway ;
>>HIF-1 signaling pathway ;
>>Autophagy - animal ;
>>mTOR signaling pathway ;
>>PI3K-Akt signaling pathway ;
>>AMPK signaling pathway ;
>>Longevity regulating pathway ;
>>Longevity regulating pathway - multiple species ;
>>TGF-beta signaling pathway ;
>>Apelin signaling pathway ;
>>Fc gamma R-mediated phagocytosis ;
>>Thermogenesis ;
>>Insulin signaling pathway ;
>>Insulin resistance ;
>>Shigellosis ;
>>Human cytomegalovirus infection ;
>>Human papillomavirus infection ;
>>Human immunodeficiency virus 1 infection ;
>>Pathways in cancer ;
>>Proteoglycans in cancer ;
>>Chemical carcinogenesis - receptor activation ;
>>Colorectal cancer ;
>>Pancreatic cancer ;
>>Acute myeloid leukemia ;
>>Breast cancer ;
>>Hepatocellular carcinoma ;
>>Gastric cancer ;
>>Choline metabolism in cancer ;
>>PD-L1 expression and PD-1 checkpoint pathway in cancer