Recommended Dilution Ratio
WB 1:1000-2000; IHC 1:200-500; IF 1:200
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Specificity
The antibody detects endogenous MAP LC3β protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Immunogen:
Recombinant Protein of MAP LC3β
Specificity:
The antibody detects endogenous MAP LC3β protein.
Protein Name:
Microtubule-associated proteins 1A/1B light chain 3B
Other Name:
MAP1LC3B ;
MAP1ALC3 ;
Microtubule-associated proteins 1A/1B light chain 3B ;
Autophagy-related protein LC3 B ;
Autophagy-related ubiquitin-like modifier LC3 B ;
MAP1 light chain 3-like protein 2 ;
MAP1A/MAP1B light chain 3 B ;
MAP1A/MAP1B LC3 B ;
Microtubule-associated protein 1 light chain 3 beta
Background:
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008],
Function:
Caution:PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.,Function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.,
Cellular Localization:
Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Mitochondrion membrane ; Lipid-anchor . Cytoplasm, cytoskeleton . Cytoplasmic vesicle . LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy (PubMed:28017329). Localizes also to discrete punctae along the ciliary axoneme. .
Tissue Expression:
Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
Research Areas:
>>Mitophagy - animal ;
>>Autophagy - animal ;
>>Ferroptosis ;
>>Apelin signaling pathway ;
>>NOD-like receptor signaling pathway ;
>>Amyotrophic lateral sclerosis ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Shigellosis ;
>>Kaposi sarcoma-associated herpesvirus infection