Target：PFK-B

Fields：Glycolysis / Gluconeogenesis;Pentose phosphate pathway;Fructose and mannose metabolism;Galactose metabolism;Metabolic pathways;Carbon metabolism;Biosynthesis of amino acids;RNA degradation;HIF-1 signaling pathway;AMPK signaling pathway;Thyroid hormone signaling pathway;Glucagon signaling pathway;Central carbon metabolism in cancer

Gene Name：PFKL

Protein Name：6-phosphofructokinase liver type

Human Gene Id：5211

Human Swiss Prot No：P17858

Mouse Gene Id：18641

Mouse Swiss Prot No：P12382

Rat Gene Id：25741

Rat Swiss Prot No：P30835

Immunogen：The antiserum was produced against synthesized peptide derived from human K6PL. AA range:691-740

Specificity：PFK-B Polyclonal Antibody detects endogenous levels of PFK-B protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PFKL;6-phosphofructokinase; liver type;Phosphofructo-1-kinase isozyme B;PFK-B;Phosphofructokinase 1;Phosphohexokinase

Observed Band(KD)：85kD

Background： This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014],

Function：catalytic activity:ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.,cofactor:Magnesium.,enzyme regulation:Allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate.,miscellaneous:In human PFK exists as a system of 3 types of subunits, PFKM (muscle), PFKL (liver) and PFKP (platelet) isoenzymes.,pathway:Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.,similarity:Belongs to the phosphofructokinase family. Two domains subfamily.,subunit:Tetramer. Muscle is M4, liver is L4, and red cell is M3L, M2L2, or ML3.,

Subcellular Location：Cytoplasm .

Expression： Brain,Cervix,Colon,Human amygdala,Human retina,Kidney,Liver,Lung,Muscle,Pla