Target：IL-1β

Fields：Antifolate resistance;MAPK signaling pathway;Cytokine-cytokine receptor interaction;NF-kappa B signaling pathway;Necroptosis;Osteoclast differentiation;Toll-like receptor signaling pathway;NOD-like receptor signaling pathway;Cytosolic DNA-sensing pathway;C-type lectin receptor signaling pathway;Hematopoietic cell lineage;IL-17 signaling pathway;Th17 cell differentiation;TNF signaling pathway;Inflammatory mediator regulation of TRP channels;Non-alcoholic fatty liver disease;AGE-RAGE signaling pathway in diabetic complications;Alcoholic liver disease;Type I diabetes mellitus;Alzheimer disease;Prion disease;Pathways of neurodegeneration - multiple diseases;Pathogenic Escherichia coli infection;Shigellosis;Salmonella infection;Pertussis;Legionellosis;Yersinia infection;Leishmaniasis;Chagas disease;African trypanosomiasis;Malaria;Amoebiasis;Tuberculosis;Measles;Human cytomegalovirus infection;Influenza A;Herpes simp

Gene Name：IL1B

Protein Name：Interleukin-1 beta

Human Gene Id：3553

Human Swiss Prot No：P01584

Mouse Gene Id：16176

Mouse Swiss Prot No：P10749

Rat Swiss Prot No：Q63264

Immunogen：The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230

Specificity：IL-1β Polyclonal Antibody detects endogenous levels of IL-1β protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：IL1B;IL1F2;Interleukin-1 beta;IL-1 beta;Catabolin

Observed Band(KD)：17kD

Background： The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008],

Function：domain:The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.,function:Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.,online information:Interleukin-1 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the IL-1 family.,subcellular location:The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.,subunit:Mono

Subcellular Location：Cytoplasm, cytosol . Secreted . Lysosome . Secreted, extracellular exosome . The precursor is cytosolic (PubMed:15192144). In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted (PubMed:24201029, PubMed:33377178, PubMed:33883744). Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744). In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation (PubMed:33883744). The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10 (PubMed:32272059). .

Expression：Expressed in activated monocytes/macrophages (at protein level).