Main Information

Target

ISG20

Host Species

Rabbit

Reactivity

Human, Mouse

Applications

WB

MW

20kD (Calculated)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of ISG20 at Human/Mouse

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Calculated)

20kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human ISG20 AA range: 87-137

Specificity:

This antibody detects endogenous levels of ISG20 at Human/Mouse

Gene Name:

ISG20 HEM45

Protein Name:

ISG20

Database Link:

Organism	Gene ID	SwissProt
Human	3669;	Q96AZ6;
Mouse	57444;	Q9JL16;

Function:

Catalytic activity:Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.,cofactor:Manganese.,Function:Exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses.,induction:Induced by interferons alpha and beta. Weaker induction was seen with interferon gamma. Increased expression was seen at the transcriptional level.,miscellaneous:Stable and constitutive expression confers resistance to vesicular stomatitis virus (VSV), influenza virus, and encephalomyocarditis virus (EMCV) infection in HeLa cells, providing an alternative antiviral pathway against RNA genomic viruses. Up regulated by E2 in estrogen receptor-positive breast cancer lines.,similarity:Belongs to the exonuclease superfamily.,subunit:Associates with PML and SP100 in the PML NB complex.,tissue specificity:Highly expressed in peripheral blood leukocytes, spleen, thymus, colon and lung.,

Cellular Localization:

Nucleus. Nucleus, nucleolus. Cytoplasm. Nucleus, Cajal body.

Tissue Expression:

Highly expressed in peripheral blood leukocytes, spleen, thymus, colon and lung. Up regulated by E2 in estrogen receptor-positive breast cancer lines.