Main Information

Target

FAK

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF, WB

MW

120kD (Observed)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous phospho levels of FAK (Phospho-Ser732) at Human:S732, Mouse:S770, Rat:S732

Purification

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

120kD

Modification

Phospho

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human FAK (Phospho-Ser732)

Specificity:

This antibody detects endogenous phospho levels of FAK (Phospho-Ser732) at Human:S732, Mouse:S770, Rat:S732

Gene Name:

PTK2 FAK FAK1

Protein Name:

FAK (Phospho-Ser732)

Other Name:

Focal adhesion kinase 1 ;
FADK 1 ;
Focal adhesion kinase-related nonkinase ;
FRNK ;
Protein phosphatase 1 regulatory subunit 71 ;
PPP1R71 ;
Protein-tyrosine kinase 2 ;
p125FAK ;
pp125FAK ;

Database Link:

Organism	Gene ID	SwissProt
Human	5747;	Q05397;

Background:

protein tyrosine kinase 2(PTK2) Homo sapiens This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. [provided by RefSeq, Oct 2015],

Function:

Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Domain:The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.,Domain:The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.,Function:Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.,PTM:Phosphorylated on 6 tyrosine residues upon activation.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.,similarity:Contains 1 FERM domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Constituent of focal adhesions.,subunit:Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB (By similarity). Interacts with TGFB1I1.,tissue specificity:Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.,

Cellular Localization:

Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus. Cytoplasm, cytoskeleton, cilium basal body . Constituent of focal adhesions. Detected at microtubules.

Tissue Expression:

Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous. Expressed in epithelial cells (at protein level) (PubMed:31630787).

Research Areas:

>>Endocrine resistance ;
>>ErbB signaling pathway ;
>>Chemokine signaling pathway ;
>>PI3K-Akt signaling pathway ;
>>Axon guidance ;
>>VEGF signaling pathway ;
>>Focal adhesion ;
>>Leukocyte transendothelial migration ;
>>Regulation of actin cytoskeleton ;
>>Growth hormone synthesis, secretion and action ;
>>Bacterial invasion of epithelial cells ;
>>Shigellosis ;
>>Yersinia infection ;
>>Amoebiasis ;
>>Human cytomegalovirus infection ;
>>Human papillomavirus infection ;
>>Human immunodeficiency virus 1 infection ;
>>Pathways in cancer ;
>>Transcriptional misregulation in cancer ;
>>Proteoglycans in cancer ;
>>Chemical carcinogenesis - reactive oxygen species ;
>>Small cell lung cancer ;
>>Lipid and atherosclerosis ;
>>Fluid shear stress and atherosclerosis