Recommended Dilution Ratio
IHC 1:50-200; WB 1:500-2000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
This antibody detects endogenous phospho levels of GRK2 (Phospho-Tyr86) at Human:Y86, Mouse:Y86, Rat:Y86
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Immunogen:
Synthesized peptide derived from human GRK2 (Phospho-Tyr86)
Specificity:
This antibody detects endogenous phospho levels of GRK2 (Phospho-Tyr86) at Human:Y86, Mouse:Y86, Rat:Y86
Gene Name:
ADRBK1 BARK BARK1 GRK2
Protein Name:
GRK2 (Phospho-Tyr86)
Other Name:
Beta-adrenergic receptor kinase 1 ;
Beta-ARK-1 ;
G-protein coupled receptor kinase 2 ;
Database Link:
| Organism |
Gene ID |
SwissProt |
| Human |
156; |
P25098; |
Background:
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008],
Function:
Catalytic activity:ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.,Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.,online information:Beta adrenergic receptor kinase entry,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,subunit:Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5.,tissue specificity:Expressed in peripheral blood leukocytes.,
Cellular Localization:
Cytoplasm . Cell membrane . Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse .
Tissue Expression:
Expressed in peripheral blood leukocytes.
Research Areas:
>>Chemokine signaling pathway ;
>>Endocytosis ;
>>Hedgehog signaling pathway ;
>>Glutamatergic synapse ;
>>Olfactory transduction ;
>>Morphine addiction
