Main Information

Target

DAPK1

Host Species

Rabbit

Reactivity

Human, Mouse

Applications

IHC, IF, WB

MW

160kD (Observed)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous phospho levels of DAPK1 (Phospho-Ser308) at Human:S308, Mouse:S308

Purification

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

160kD

Modification

Phospho

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human DAPK1 (Phospho-Ser308)

Specificity:

This antibody detects endogenous phospho levels of DAPK1 (Phospho-Ser308) at Human:S308, Mouse:S308

Gene Name:

DAPK1 DAPK

Protein Name:

DAPK1 (Phospho-Ser308)

Other Name:

Death-associated protein kinase 1 ;
DAP kinase 1 ;

Database Link:

Organism	Gene ID	SwissProt
Human	1612;	P53355;

Background:

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],

Function:

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-308.,Function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,induction:Up-regulated following treatment with interferon-gamma.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 10 ANK repeats.,subcellular location:Colocalizes with the actin filament system.,

Cellular Localization:

[Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton.

Tissue Expression:

Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.

Research Areas:

>>Autophagy - animal ;
>>Pathways in cancer ;
>>Bladder cancer