Main Information

Target

Caspase-10

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

MW

58kD,23kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000; ELISA(peptide)1:5000-20000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous levels of human Caspase 10 (p23/17, Cleaved-Val220)

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

58kD,23kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human Caspase 10 (p23/17, Cleaved-Val220)

Specificity:

This antibody detects endogenous levels of human Caspase 10 (p23/17, Cleaved-Val220)

Gene Name:

CASP10 MCH4

Protein Name:

Caspase10

Other Name:

Caspase-10 ;
CASP-10 ;
Apoptotic protease Mch-4 ;
FAS-associated death domain protein interleukin-1B-converting enzyme 2 ;
FLICE2 ;
ICE-like apoptotic protease 4 ;
[Cleaved into: Caspase-10 subunit p23/17 ;
Caspase-10 subunit p12]

Database Link:

Organism	Gene ID	SwissProt
Human	843;	Q92851;

Background:

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011],

Function:

Catalytic activity:Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Leu-Gln-Thr-Asp-|-Gly.,Disease:Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.,Disease:Defects in CASP10 are a cause of gastric cancers [MIM:137215].,Disease:Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.,Function:Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.,Function:Isoform C is proteolytically inactive.,online information:CASP10 mutation db,online information:Caspase-10 mutations causing ALPS type II,PTM:Cleavage by granzyme B and autocatalytic activity generate the two active subunits.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 2 DED (death effector) domains.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit (By similarity). Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10.,tissue specificity:Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.,

Cellular Localization:

cytosol,CD95 death-inducing signaling complex,ripoptosome,

Tissue Expression:

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

Research Areas:

>>Apoptosis ;
>>RIG-I-like receptor signaling pathway ;
>>TNF signaling pathway ;
>>Tuberculosis ;
>>Hepatitis B