Main Information

Target

AMPKβ2

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB

MW

30kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-2000

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous AMPKβ2 protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

30kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of AMPKβ2

Specificity:

The antibody detects endogenous AMPKβ2 protein.

Gene Name:

PRKAB2

Protein Name:

5'-AMP-activated protein kinase subunit beta-2

Other Name:

PRKAB2 ;
5'-AMP-activated protein kinase subunit beta-2 ;
AMPK subunit beta-2

Database Link:

Organism	Gene ID	SwissProt
Human	5565;	O43741;
Mouse		Q6PAM0;
Rat		Q9QZH4;

Background:

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013],

Function:

Function:AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. Also regulates cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase and hormone-sensitive lipase. This is a regulatory subunit, may be a positive regulator of AMPK activity. It may also serve as an adapter molecule for the catalytic alpha-subunit.,PTM:Phosphorylated when associated with the catalytic subunit.,similarity:Belongs to the 5'-AMP-activated protein kinase beta subunit family.,subunit:Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic regulatory subunits.,

Cellular Localization:

nucleoplasm,cytosol,nucleotide-activated protein kinase complex,

Tissue Expression:

Liver,Pancreas,

Research Areas:

>>FoxO signaling pathway ;
>>AMPK signaling pathway ;
>>Longevity regulating pathway ;
>>Longevity regulating pathway - multiple species ;
>>Apelin signaling pathway ;
>>Tight junction ;
>>Circadian rhythm ;
>>Thermogenesis ;
>>Insulin signaling pathway ;
>>Adipocytokine signaling pathway ;
>>Oxytocin signaling pathway ;
>>Glucagon signaling pathway ;
>>Insulin resistance ;
>>Non-alcoholic fatty liver disease ;
>>Alcoholic liver disease ;
>>Hypertrophic cardiomyopathy