Main Information

Target

HSC70

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

MW

70-72kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1:2000; IHC:1:50-300; IF 1:50-200

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous HSC 70 protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

70-72kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from the Internal region of human HSC 70. AA range: 588-638

Specificity:

The antibody detects endogenous HSC 70 protein.

Gene Name:

HSPA8

Protein Name:

Heat shock cognate 71 kDa protein

Other Name:

HSPA8 ;
HSC70 ;
HSP73 ;
HSPA10 ;
Heat shock cognate 71 kDa protein ;
Heat shock 70 kDa protein 8

Database Link:

Organism	Gene ID	SwissProt
Human	3312;	P11142;
Mouse		P63017;
Rat		P63018;

Background:

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],

Function:

Function:Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.,induction:Constitutively synthesized.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 70 family.,subcellular location:Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with HSPH1/HSP105. Interacts with IRAK1BP1 (By similarity). Interacts with PACRG and TSC2. Interacts with SV40 VP1.,tissue specificity:Ubiquitous.,

Cellular Localization:

Cytoplasm. Melanosome. Nucleus, nucleolus. Cell membrane. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock.

Tissue Expression:

Ubiquitous.

Research Areas:

>>Spliceosome ;
>>MAPK signaling pathway ;
>>Protein processing in endoplasmic reticulum ;
>>Endocytosis ;
>>Longevity regulating pathway - multiple species ;
>>Antigen processing and presentation ;
>>Estrogen signaling pathway ;
>>Prion disease ;
>>Legionellosis ;
>>Toxoplasmosis ;
>>Measles ;
>>Lipid and atherosclerosis