Main Information

Target

NBR1

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

MW

120kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:50-300; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

The antibody detects endogenous NBR1 protein

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

120kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of NBR1

Specificity:

The antibody detects endogenous NBR1 protein

Gene Name:

NBR1 1A13B KIAA0049 M17S2 MIG19

Protein Name:

Next to BRCA1 gene 1 protein (Cell migration-inducing gene 19 protein) (Membrane component chromosome 17 surface marker 2) (Neighbor of BRCA1 gene 1 protein) (Protein 1A1-3B)

Other Name:

Next to BRCA1 gene 1 protein ;
Cell migration-inducing gene 19 protein ;
Membrane component chromosome 17 surface marker 2 ;
Neighbor of BRCA1 gene 1 protein ;
Protein 1A1-3B ;

Database Link:

Organism	Gene ID	SwissProt
Human	4077;	Q14596;
Mouse		P97432;
Rat		Q501R9;

Background:

The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014],

Function:

Caution:Was originally (PubMed:8069304) thought to be the ovarian carcinoma antigen CA125.,Domain:The OPR domain mediates interaction with SQSTM1.,similarity:Contains 1 OPR domain.,similarity:Contains 1 UBA domain.,similarity:Contains 1 ZZ-type zinc finger.,subunit:Homooligomer and heterooligomer. Interacts with SQSTM1, titin/TTN and RNF29.,

Cellular Localization:

Cytoplasm . Cytoplasmic vesicle, autophagosome . Lysosome . Cytoplasm, myofibril, sarcomere, M line . In cardiac muscles localizes to the sarcomeric M line (By similarity). Is targeted to lysosomes for degradation (PubMed:19250911). .

Tissue Expression:

Bone marrow,Lymph,Ovary,Testis,

Research Areas:

>>Mitophagy - animal