产品简介：
pMAL-c5X is an E. coli plasmid cloning vector designed for recombinant protein expression and purification using the pMAL Protein Fusion and Purification System. It contains the pMB1 origin of replication from pBR322 and is maintained at a similar copy number to pBR322. The multiple cloning site (MCS) is positioned to allow translational fusion of the E. coli maltose binding protein (MBP, encoded by the malE gene) to the N-terminus of the cloned target protein. The pMAL-p5 and -c5 series of vectors differs from the -p4 and -c4 series in that they contain a universal multiple cloning site (MCS) that is compatible with other NEB expression systems and is followed by stop codons in all three reading frames. In addition, lacZα and the M13 origin have been removed. In these vectors, MBP has been engineered for tighter binding to amylose. This allows easy purification of the fusion protein, and the MBP domain can be subsequently removed using Factor Xa protease. pMAL-c5-series vectors are identical to the pMAL-p5-series vectors above except for a deletion of the malE signal sequence.

 

产品参数：
出品公司：NEB
克隆方法：多克隆位点，限制性内切酶
启动子：Ptac
载体大小：5677 bp
载体抗性：Ampicillin
诱导方式：IPTG或乳糖及其类似物

关键功能：融合表达MBP蛋白

 

 

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。