产品简介：
KRX is an E. coli K12 derivative that has salient features associated with cloning and screening strains plus engineered attributes to optimize controlled protein expression. This cloning strain is the partially defective in hsd, e14, endA–, and recA– systems. KRX can be used for blue/white screening because it is deficient in β-galactosidase activity due to deletions in both genomic and episomal copies of the lacZ gene. KRX also is a good expression strain. The ompT– and ompP– mutations eliminate one source of proteolysis of overexpressed protein in E. coli. KRX incorporates a chromosomal copy of the T7 RNA polymerase gene driven by the rhamnose promoter (rhaPBAD) to provide dramatic control of recombinant protein expression. This promoter is subject to catabolite repression by glucose and activated by adding rhamnose to the medium, thus providing precise control of T7 RNA polymerase abundance and thereby precise control of recombinant protein production.

产品参数：
出品公司：Promega

载体类型：原核表达系统
基因型：

 

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。