产品简介：
The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII. The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus. pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).

 

产品参数：
出品公司：MoBiTec GmbH

质粒类型：乳酸杆菌表达载体；乳酸乳球菌表达载体

启动子: PnisA

复制子: repA，repC

终止子: T

载体大小：3167bp

载体抗性：Chloramphenicol

筛选标记：Chloramphenicol

克隆菌株：MC1061

培养条件：M17+0.5%葡萄糖/30度密封静置液体培养

表达宿主：NZ9000乳酸乳球菌

5' 测序引物及序列：根据序列设计引物
3' 测序引物及序列：根据序列设计引物

备注：注意pNZ8148转化扩增、克隆构建时必须使用MC1061感受态。您可以从HonorGene购买获得MC1061菌株。

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。