产品简介：
pCold TF DNA Vector is a fusion cold shock expression vector that expresses Trigger Factor (TF) chaperone as a soluble tag. Trigger Factor is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed polypeptides. Because of its E. coli origin, TF is highly expressed in E. coli expression systems. The pCold TF DNAVector consists of the csp A promoter plus additional downstream sequences including a 5' untranslated region (5' UTR), a translation enhancing element (TEE), a His-Tag sequence, and a multicloning site (MCS). A lac operator is inserted downstream of the csp A promoter to ensure strict regulation of expression. Additionally, recognition sites for HRV 3C Protease, Thrombin, and Factor Xa are located between TF-Tag and the Multiple Cloning Site (MCS) and function to facilitate tag removal from the expressed fusion protein.

 

 

产品参数：

质粒类型：大肠杆菌冷激表达载体

出品公司：Takara

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：5769 bp

5' 测序引物及序列：pCold-TF-F1: 5'-CCACTTTCAACGAGCTGATG-3';
pCold-TF-F2: 5'-GCGAAAGTGACTGAAAAAG-3'
3' 测序引物及序列：pCold-R: 5'-GGCAGGGATCTTAGATTCTG-3'
载体标签：N-His, N-Trigger factor, N-HRV 3C, N-Thrombin, N-Factor Xa
载体抗性：Ampicillin (氨苄青霉素)
备注:：温度诱导，冷休克蛋白表达载体

 

 

 

 

 

 

 

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。