产品简介：
Cold-shock expression vectors, pCold DNA I-IV, are designed to perform efficient protein expression utilizing the promoter derived from cspA gene, which is one of the cold-shock genes. At the downstream of the cspA promoter, lac operator is inserted so that the expression is strictly controlled. In addition, 5' untranslated region (5' UTR), translation enhancing element(TEE), His-Tag sequence, Factor Xa cleavage site, and multicloning site(MCS) are located at the downstream of the cspA promoter. As this product utilizes the promoter derived from E.coli, most E.coli strains can be utilized as an expression host. There are four kinds of pCold vectors, whose arrangements vary in the existence of TEE, His Tag Sequence and Factor Xa clevage site.

 

产品参数：

质粒类型：大肠杆菌冷激表达载体

出品公司：Takara

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：4377 bp

5' 测序引物及序列：pCold-F: 5'-ACGCCATATCGCCGAAAGG-3'
3' 测序引物及序列：pCold-R: 5'-GGCAGGGATCTTAGATTCTG-3'
载体标签：无
载体抗性：Ampicillin (氨苄青霉素)
备注:：温度诱导，冷休克蛋白表达载体

 

 

 

 

 

 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。