产品简介:
BL21(DE3) is an exemplary host for the expression of recombinant proteins. The strain carries a deletion in the ompT gene which encodes an outer membrane protease shown to degrade recombinant products. As an E. coli B strain, it also lacks a major protease, encoded by the lon gene, which catalyzes the endoproteolytic cleavage of damaged and recombinant proteins in the cell. The BL21(DE3) is an all purpose strain for high-level protein expression and easy induction. When used with the CE6 bacteriophage, the BL21(DE3) provides the tightest control of protein expression.
产品参数:
出品公司:Novagen
抗性:无载体类型:原核表达载体
基因型:F- ompT hsdS(rB-mB-)gal dcm(DE3)
菌株类型:E.coli
培养条件:37℃,有氧,LB
质粒转化:42℃热激
诱导方式:IPTG或乳糖及其类似物
保存方式:20%甘油,-20℃
注意事项:
1、为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解,在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用,菌体会逐渐沉淀至管底,请混匀后使用。随着冻融次数的增加,菌株的活力会逐渐下降。