产品简介：
pCOLADuet-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His•Tag sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S•Tag™ peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to 8 target genes.

 

产品参数：
质粒类型：大肠杆菌表达载体（双启动子）
表达水平：高
克隆方法：多克隆位点，限制性内切酶
载体大小：3719 bp
5' 测序引物及序列：T7：5'-TAATACGACTCACTATAGGG-3';
3' 测序引物及序列：T7t：5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签：N-His, C-S
载体抗性：Kanamycin (卡那霉素)
备注：pRSFDuet-1 carries the RSF1030 replicon, lacI gene and kanamycin resistance gene. 

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。