产品简介:
pACYCDuet™-1 is designed for the coexpression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and ribosome binding site (rbs). The vector also carries the P15A replicon, lacI gene and chloramphenicol resistance gene. This vector can be used in combination with pETDuet™-1 in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer 。 Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer 。
产品参数:
质粒类型:大肠杆菌表达载体(双启动子)
表达水平:高
克隆方法:多克隆位点,限制性内切酶
载体大小:4008 bp
5' 测序引物及序列:T7: 5'-TAATACGACTCACTATAGGG-3';
3' 测序引物及序列:T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签:N-His, C-S
载体抗性:氯霉素(Chloramphenicol)
备注:pACYCDuet-1 is designed for the coexpression of two target genes. pACYCDuet-1 carries the P15A replicon, lacI gene and chloramphenicol resistance gene.注意事项:
1、为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解,在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用,菌体会逐渐沉淀至管底,请混匀后使用。随着冻融次数的增加,菌株的活力会逐渐下降。