产品简介：
pETDuet-1 is designed for the coexpression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and a ribosome binding site (rbs). The vector also carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene. This vector can be used in combination with pACYCDuet™-1 in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the pET Upstream Primer and DuetDOWN1 Primer 。Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer。

产品参数：
质粒类型：大肠杆菌表达载体（双启动子）
表达水平：高
克隆方法：多克隆位点，限制性内切酶
载体大小：5420 bp 
5' 测序引物及序列：T7: 5'-TAATACGACTCACTATAGGG-3';
3' 测序引物及序列：T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签：N-His, C-S
载体抗性：Ampicillin (氨苄青霉素)
备注：pETDuet-1 is designed for the coexpression of two target genes. pETDuet-1 carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene.

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。