产品简介：
The pET-52b(+) vector carries an N-terminal Strep•Tag® II coding sequence (1) followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ↓GP (2), and is active at low temperatures (3). The multiple cloning region is followed by an optional thrombin recognition site and a C-terminal His•Tag® coding sequence. The presence of two “gentle elution" tags at both the N-terminus and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (4). Unique restriction sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below the circle map. The f1 origin is oriented so that infection with the helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand。

 

产品参数：

载体名称：pET-52b, pET52b

质粒类型：大肠杆菌表达载体；蛋白纯化

高拷贝/低拷贝：低拷贝

克隆方法：多克隆位点；限制性内切酶

启动子：T7

载体大小：5227 bp

5' 测序引物及序列：T7 Fwd：5'd[TAATACGACTCACTATAGGG]3'

载体标签：His Tag (C-末端)；Strep TagII (N-末端)

载体抗性：氨苄青霉素（Ampicillin）

备注：Strep TagII 下游含HRV 3C识别位点；
His Tag 上游含Thrombin识别位点

稳定性：稳表达

组成型/诱导型：诱导型（IPTG)

病毒/非病毒：非病毒

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。