产品简介：

 

The pAd⁄CMV⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄CMV⁄V5-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter.

 

Advantages

• High efficiency and rapid recombination cloning

• Produces high titer adenoviral stocks

• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo

• Produces replication-incompetent virus for enhanced biosafety of the system

• Amenable for use in high-throughput applications

 

Key Features

• Gateway® Technology for efficient and rapid cloning

• CMV promoter for high-level constitutive expression of gene of interest

• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the

 

质粒类型:    腺病毒载体

启动子:    CMV

克隆方法:    Gateway

载体大小:    36686 bp

5' 测序引物及序列:    CMVPro Fwd: 5'd[CGCAAATGGGCGGTAGGCGTG]3'

载体标签:    V5

载体抗性:    Ampicillin (氨苄青霉素)