产品简介：

 

The pAd⁄PL-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄PL-DEST® vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.

 

Advantages

• High efficiency and rapid recombination cloning

• Produces high titer adenoviral stocks

• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo

• Allows gene of interest to be controlled by a promoter of choice

• Produces replication-incompetent virus for enhanced biosafety of the system

• Amenable for use in high-throughput applications

 

Key Features

• Gateway® Technology for efficient and rapid cloning

• Promoterless vector that allows gene of interest to be controlled by a promoter of choice

• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions

 

 

质粒类型:    腺病毒载体

启动子:    无启动子

克隆方法:    Gateway Cloning

载体大小:    34864 bp 

载体抗性:    Ampicillin，Chloramphenicol