pGL4.19[lucCP/Neo] 载体
质粒类型: 启动子报告载体
克隆方法: 多克隆位点，限制性内切酶
载体大小: 5778 bp (查看载体序列)
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Neomycin (新霉素)
备注: Promoterless vector encoding highly destabilized luciferase for measuring the activity of promoter and enhancer sequences. 

载体描述
The pGL4.19[luc2CP /Neo] Vector(a–e) encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression.

The pGL4.19[luc2CP /Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The luc2CP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by luc2CP responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.