产品简介:
pTet-Off expresses the tet-responsive transcriptional activator (tTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-Off was originally described as pUHD15-1neo by Resnitzky et al. (1994) and was created by insertion of a neomycin resistance gene into pUHD15-1 (Gossen & Bujard, 1992). pTet-Off can be distinguished from pTet-On by digestion with Hind III.
产品参数:
质粒类型:四环素调控系统
出品公司:Clontech
启动子:CMV
克隆方法:多克隆位点,限制性内切酶
载体大小:7391 bp
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:Neomycin (新霉素)