载体介绍:
pSilencer 5.1 Retro vectors harness retroviruses’ ability to stably integrate into the host genome of nearly all mitotically active cells and include a puromycin resistance gene for selection of cells that express the recombinant DNA. The pSilencer 5.1 Retro vectors enable researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. This technology expands the capabilities of RNA interference research to address long-term alteration of gene expression.
产品参数:
质粒类型:RNAi载体
高拷贝/低拷贝:低拷贝
启动子:U6
克隆方法:多克隆位点,限制性内切酶
载体大小:6486bp
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:Puromycin(嘌呤霉素)
备注:逆病毒基因沉默载体,能够将发卡的siRNA转染进入大多数哺乳细胞中