产品简介：

The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae. Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter. These vectors also feature an extensive polylinker sequence and the ability to generate end-specific RNA transcripts from T3 and T7 promoters. Each of the pESC vectors contains one of four different yeast-selectable markers (HIS3, TRP1, LEU2, or URA3) in the same vector backbone.

The pESC vectors contain DNA sequences coding for epitope peptides that can be specifically recognized by monoclonal antibodies. A sequence for the FLAG epitope (DYKDDDDK) is located in the multiple cloning site (MCS) downstream of the GAL10 promoter；a sequence for the c-Myc epitope (EQKLISEEDL) is located in the MCS downstream of the GAL1 promoter. You can insert your gene of interest in front of the epitope sequence to generate C-terminal tagging or after the epitope sequence for N-terminal tagging. These tags allow the protein of interest to be studied without generating a specific antibody to that protein. The epitope-tagged fusion proteins can be studied in transformed cells using well-characterized antibodies.

Express two different genes simultaneously in S. cerevisiae； 

Proteins tagged with unique epitope tags；

Fast and easy immunoprecipitations.

 

产品参数：

载体用途：酵母表达载体（双表达框）；酿酒酵母表达载体（双表达框）

出品公司：Stratagene

质粒大小：6525bp

原核抗性：Amp+

筛选标记：TRP1

启动子：GAL1 promoter，GAL10 promoter

质粒标签：C-Flag, C-Myc

克隆方法：多克隆位点，限制性内切酶

克隆菌株：DH5a

培养条件：37℃

表达宿主：酿酒酵母细胞

表达诱导方式：半乳糖诱导

5'测序引物：GAL1-F（5'-ATTTTCGGTTTGTATTACTTC-3'）；GAL10-F（5'-GGTGGTAATGCCATGTAATATG-3'）

3'测序引物：GAL1-R（5'-GTTCTTAATACTAACATAACT-3'）；GAL10-R（5'-GGCAAGGTAGACAAGCCGACAAC-3'）

备注：利用半乳糖诱导，可以同时在酿酒酵母中表达两个基因。