载体介绍:

RNAi-Ready pSIREN-RetroQ-DsRed-Express is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter (PU6; RNA Pol III-dependent). RNAi-Ready pSIREN-RetroQ-DsRed-Express is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when a ds DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQDsRed-Express construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA.

产品参数:

质粒类型：RNAi载体

高拷贝/低拷贝：低拷贝

启动子：Human U6

克隆方法：多克隆位点，限制性内切酶

载体大小：6543 bp

5' 测序引物及序列：U6-F:ATGGACTATCATATGCTTACCGTA (Clontech )

载体抗性：Ampicillin (氨苄青霉素)

备注：载体表达DsRed红色荧光蛋白作为基因沉默标志。