Target：CaMKIα
Fields：Calcium signaling pathway;Oxytocin signaling pathway;Aldosterone synthesis and secretion;Glioma
Gene Name：CAMK1
Protein Name：Calcium/calmodulin-dependent protein kinase type 1
Human Gene Id：8536
Human Swiss Prot No：Q14012
Mouse Gene Id：52163
Mouse Swiss Prot No：Q91YS8
Rat Gene Id：171503
Rat Swiss Prot No：Q63450
Immunogen：The antiserum was produced against synthesized peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. AA range:143-192
Specificity：Phospho-CaMKIα (T177) Polyclonal Antibody detects endogenous levels of CaMKIα protein only when phosphorylated at T177.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：CAMK1;Calcium/calmodulin-dependent protein kinase type 1;CaM kinase I;CaM-KI;CaM kinase I alpha;CaMKI-alpha
Observed Band(KD)：41kD
Background： Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin results in a conformational change that generates functional binding sites for both, substrate and ATP, and thus releaves intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium-dependent activation of the ERK pathway (By si
Subcellular Location：Cytoplasm . Nucleus . Predominantly cytoplasmic. .
Expression：Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
