Target：EphB1/2
Fields：Axon guidance
Gene Name：EPHB1/EPHB2
Protein Name：Ephrin type-B receptor 1/2
Human Gene Id：2047/1969
Human Swiss Prot No：P54762/P29323
Mouse Gene Id：270190
Rat Gene Id：24338
Rat Swiss Prot No：P09759
Immunogen：The antiserum was produced against synthesized peptide derived from human EPHB1/2 around the phosphorylation site of Tyr594/604. AA range:561-610
Specificity：Phospho-EphB1/2 (Y594/604) Polyclonal Antibody detects endogenous levels of EphB1/2 protein only when phosphorylated at Y594/604.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：EPHB1;ELK;EPHT2;HEK6;NET;Ephrin type-B receptor 1;ELK;EPH tyrosine kinase 2;EPH-like kinase 6;EK6;hEK6;Neuronally-expressed EPH-related tyrosine kinase;NET;Tyrosine-protein kinase receptor EPH-2;EPHB2;DRT;EPHT3;EPTH3;ERK;
Observed Band(KD)：110kD
Background： Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. May be involved in cell-cell interactions in the nervous system.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,subunit:The ligand-activated form interacts with GRB2, GRB10 and NCK through their respective SH2 domains. The GRB10 SH2 domain binds EPHB1 through Tyr-928, while GRB2 binds residues within the catalytic domain. Interacts with EPHB6. The NCK SH2 domain binds EPHB1 through Tyr-594. Interacts with PRKCABP.,tissue specificity:Preferentially expressed in brain.,
Subcellular Location：Cell membrane ; Single-pass type I membrane protein . Early endosome membrane . Cell projection, dendrite .
Expression：Preferentially expressed in brain.
