Target：DARPP-32

Fields：cAMP signaling pathway;Dopaminergic synapse;Cocaine addiction;Amphetamine addiction;Alcoholism

Gene Name：PPP1R1B

Protein Name：Protein phosphatase 1 regulatory subunit 1B

Human Gene Id：84152

Human Swiss Prot No：Q9UD71

Mouse Gene Id：19049

Mouse Swiss Prot No：Q60829

Rat Gene Id：360616

Rat Swiss Prot No：Q6J4I0

Immunogen：The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr34. AA range:18-67

Specificity：Phospho-DARPP-32 (T34) Polyclonal Antibody detects endogenous levels of DARPP-32 protein only when phosphorylated at T34.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PPP1R1B;DARPP32;Protein phosphatase 1 regulatory subunit 1B;DARPP-32;Dopamine- and cAMP-regulated neuronal phosphoprotein

Observed Band(KD)：35kD

Background： This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],

Function：function:Inhibitor of protein-phosphatase 1.,PTM:Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.,PTM:Phosphorylation of Thr-34 is required for activity.,similarity:Belongs to the protein phosphatase inhibitor 1 family.,

Subcellular Location：Cytoplasm.

Expression： Adipose tissue,Brain,Cerebellum,Colon,Ovary,