Target：DAPK3
Fields：Autophagy - animal;Pathways in cancer;Bladder cancer
Gene Name：DAPK3
Protein Name：Death-associated protein kinase 3
Human Gene Id：1613
Human Swiss Prot No：O43293
Mouse Gene Id：13144
Mouse Swiss Prot No：O54784
Rat Gene Id：64391
Rat Swiss Prot No：O88764
Immunogen：The antiserum was produced against synthesized peptide derived from human DAPK3 around the phosphorylation site of Thr265. AA range:241-290
Specificity：Phospho-DAPK3 (T265) Polyclonal Antibody detects endogenous levels of DAPK3 protein only when phosphorylated at T265.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：DAPK3;ZIPK;Death-associated protein kinase 3;DAP kinase 3;DAP-like kinase;Dlk;MYPT1 kinase;Zipper-interacting protein kinase;ZIP-kinase
Observed Band(KD)：52kD
Background： Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Associates to centromeres from prophase to anaphase.,subunit:Homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. Interacts with AATF and CDC5L.,
Subcellular Location：Nucleus . Cytoplasm . Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. .; [Isoform 1]: Nucleus . Cytoplasm .; [Isoform 2]: Nucleus . Cytoplasm .
Expression：Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle.
