Target：IL-4R/CD124
Fields：Cytokine-cytokine receptor interaction;PI3K-Akt signaling pathway;JAK-STAT signaling pathway;Hematopoietic cell lineage;Th1 and Th2 cell differentiation;Th17 cell differentiation;Pathways in cancer;Inflammatory bowel disease
Gene Name：IL4R
Protein Name：Interleukin-4 receptor subunit alpha
Human Gene Id：3566
Human Swiss Prot No：P24394
Mouse Gene Id：16190
Mouse Swiss Prot No：P16382
Immunogen：The antiserum was produced against synthesized peptide derived from human IL-4R/CD124 around the phosphorylation site of Tyr497. AA range:463-512
Specificity：Phospho-IL-4Rα (Y497) Polyclonal Antibody detects endogenous levels of IL-4Rα protein only when phosphorylated at Y497.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：IL4R;IL4RA;582J2.1;Interleukin-4 receptor subunit alpha;IL-4 receptor subunit alpha;IL-4R subunit alpha;IL-4R-alpha;IL-4RA;CD antigen CD124
Observed Band(KD)：90kD
Background： This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012],
Function：domain:Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.,domain:The box 1 motif is required for JAK interaction and/or activation.,domain:The extracellular domain represents the IL4 binding protein (IL4BP).,domain:The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.,function:Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cel
Subcellular Location：Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
Expression：Isoform 1 and isoform 2 are highly expressed in activated T-cells.
