Target:NOS3
Fields:Arginine biosynthesis;Arginine and proline metabolism;Metabolic pathways;Calcium signaling pathway;cGMP-PKG signaling pathway;HIF-1 signaling pathway;Sphingolipid signaling pathway;PI3K-Akt signaling pathway;VEGF signaling pathway;Apelin signaling pathway;Platelet activation;Estrogen signaling pathway;Oxytocin signaling pathway;Relaxin signaling pathway;Insulin resistance;AGE-RAGE signaling pathway in diabetic complications;Diabetic cardiomyopathy;Lipid and atherosclerosis;Fluid shear stress and atherosclerosis
Gene Name:NOS3
Protein Name:Nitric oxide synthase endothelial
Human Gene Id:4846
Human Swiss Prot No:P29474
Mouse Gene Id:18127
Mouse Swiss Prot No:P70313
Rat Gene Id:24600
Rat Swiss Prot No:Q62600
Immunogen:The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser615. AA range:581-630
Specificity:Phospho-NOS3 (S615) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at S615.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:NOS3;Nitric oxide synthase; endothelial;Constitutive NOS;cNOS;EC-NOS;Endothelial NOS;eNOS;NOS type III;NOSIII
Observed Band(KD):130-140kD
Background: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
Function:catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like
Subcellular Location:Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Expression:Platelets, placenta, liver and kidney.