Target：NIFK
Gene Name：MKI67IP
Protein Name：MKI67 FHA domain-interacting nucleolar phosphoprotein
Human Gene Id：84365
Human Swiss Prot No：Q9BYG3
Mouse Gene Id：67949
Mouse Swiss Prot No：Q91VE6
Immunogen：The antiserum was produced against synthesized peptide derived from human NIFK around the phosphorylation site of Thr234. AA range:200-249
Specificity：Phospho-NIFK (T234) Polyclonal Antibody detects endogenous levels of NIFK protein only when phosphorylated at T234.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：MKI67IP;NIFK;NOPP34;MKI67 FHA domain-interacting nucleolar phosphoprotein;Nucleolar phosphoprotein Nopp34;Nucleolar protein interacting with the FHA domain of pKI-67;hNIFK
Molecular Weight(Da)：34kD
Background： This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009],
Function：PTM:Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Localizes to mitotic chromosomes in conjunction with MKI67.,subunit:Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.,
Subcellular Location：Nucleus, nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
Expression： Brain,Cervix carcinoma,Epithelium,Lung,Plac
