Target：Nur77

Fields：MAPK signaling pathway;PI3K-Akt signaling pathway;Aldosterone synthesis and secretion;Cortisol synthesis and secretion;Cushing syndrome

Gene Name：NR4A1

Protein Name：Nuclear receptor subfamily 4 group A member 1

Human Gene Id：3164

Human Swiss Prot No：P22736

Mouse Gene Id：15370

Mouse Swiss Prot No：P12813

Rat Gene Id：79240

Rat Swiss Prot No：P22829

Immunogen：The antiserum was produced against synthesized peptide derived from human Nuclear Receptor NR4A1 around the phosphorylation site of Ser351. AA range:317-366

Specificity：Phospho-Nur77 (S351) Polyclonal Antibody detects endogenous levels of Nur77 protein only when phosphorylated at S351.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:40000. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：NR4A1;GFRP1;HMR;NAK1;Nuclear receptor subfamily 4 group A member 1;Early response protein NAK1;Nuclear hormone receptor NUR/77;Nur77;Orphan nuclear receptor HMR;Orphan nuclear receptor TR3;ST-59;Testicular receptor 3

Observed Band(KD)：65-70kD

Background： This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011],

Function：function:Orphan nuclear receptor.,induction:By growth-stimulating agents.,similarity:Belongs to the nuclear hormone receptor family.,similarity:Belongs to the nuclear hormone receptor family. NR4 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Interacts with GADD45GIP1.,tissue specificity:Fetal muscle and adult liver, brain and thyroid.,

Subcellular Location：Nucleus . Cytoplasm . Mitochondrion . Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27 (PubMed:22427340). Translocation to the mitochondrion upon interaction with RXRA and upon the presence of 9-cis retinoic acid (PubMed:17761950). .

Expression：Fetal muscle and adult liver, brain and thyroid.