Target：p130 Cas
Fields：Rap1 signaling pathway;Chemokine signaling pathway;Focal adhesion;Leukocyte transendothelial migration;Regulation of actin cytoskeleton;Growth hormone synthesis, secretion and action;Bacterial invasion of epithelial cells;Shigellosis;Yersinia infection;Human cytomegalovirus infection
Gene Name：BCAR1
Protein Name：Breast cancer anti-estrogen resistance protein 1
Human Gene Id：9564
Human Swiss Prot No：P56945
Mouse Gene Id：12927
Mouse Swiss Prot No：Q61140
Rat Gene Id：25414
Rat Swiss Prot No：Q63767
Immunogen：The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr165. AA range:131-180
Specificity：Phospho-p130 Cas (Y165) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y165.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：BCAR1;CAS;CASS1;CRKAS;Breast cancer anti-estrogen resistance protein 1;CRK-associated substrate;Cas scaffolding protein family member 1;p130cas
Observed Band(KD)：130kD
Background： BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009],
Function：domain:A serine-rich region promotes activation of the serum response element (SRE).,domain:Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL.,domain:The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.,function:Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.,PTM:Focal adhesion kinase 1 phosphoryl
Subcellular Location：Cell junction, focal adhesion . Cytoplasm . Cell projection, axon . Unphosphorylated form localizes in the cytoplasm (By similarity). Localizes to focal adhesion sites following integrin engagement (By similarity). .
Expression：Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
