Target：HSC 70

Fields：Spliceosome;MAPK signaling pathway;Protein processing in endoplasmic reticulum;Endocytosis;Longevity regulating pathway - multiple species;Antigen processing and presentation;Estrogen signaling pathway;Prion disease;Legionellosis;Toxoplasmosis;Measles;Lipid and atherosclerosis

Gene Name：HSPA8

Protein Name：Heat shock cognate 71 kDa protein

Human Gene Id：3312

Human Swiss Prot No：P11142

Mouse Gene Id：15481

Mouse Swiss Prot No：P63017

Rat Gene Id：24468

Rat Swiss Prot No：P63018

Immunogen：The antiserum was produced against synthesized peptide derived from human HSC 70. AA range:202-251

Specificity：HSC 70 Polyclonal Antibody detects endogenous levels of HSC 70 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：HSPA8;HSC70;HSP73;HSPA10;Heat shock cognate 71 kDa protein;Heat shock 70 kDa protein 8

Observed Band(KD)：70kD

Background： This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],

Function：function:Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.,induction:Constitutively synthesized.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 70 family.,subcellular location:Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with HSPH1/HSP105. Interacts with IRAK1BP1 (By similarity). Interacts with PACRG and TSC2. Interacts with SV40 VP1.,tissue specificity:Ubiquitous.,

Subcellular Location：Cytoplasm. Melanosome. Nucleus, nucleolus. Cell membrane. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock.

Expression：Ubiquitous.