Target：RUNX3

Fields：Th1 and Th2 cell differentiation;Epstein-Barr virus infection

Gene Name：RUNX3

Protein Name：Runt-related transcription factor 3

Human Gene Id：864

Human Swiss Prot No：Q13761

Mouse Swiss Prot No：Q64131

Immunogen：The antiserum was produced against synthesized peptide derived from human RUNX3. AA range:133-182

Specificity：RUNX3 Polyclonal Antibody detects endogenous levels of RUNX3 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：RUNX3;AML2;CBFA3;PEBP2A3;Runt-related transcription factor 3;Acute myeloid leukemia 2 protein;Core-binding factor subunit alpha-3;CBF-alpha-3;Oncogene AML-2;Polyomavirus enhancer-binding protein 2 alpha C subunit;PEA2-alpha C;PEB

Observed Band(KD)：44kD

Background： This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],

Function：domain:A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.,function:CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.,similarity:Contains 1 Runt domain.,subunit:Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1.,

Subcellular Location：Nucleus . Cytoplasm . The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation. .

Expression：Expressed in gastric cancer tissues (at protein level).