Target：GRK 5

Fields：Chemokine signaling pathway;Endocytosis;Morphine addiction

Gene Name：GRK5

Protein Name：G protein-coupled receptor kinase 5

Human Gene Id：2869

Human Swiss Prot No：P34947

Mouse Gene Id：14773

Mouse Swiss Prot No：Q8VEB1

Rat Gene Id：59075

Rat Swiss Prot No：Q62833

Immunogen：The antiserum was produced against synthesized peptide derived from human GRK5. AA range:351-400

Specificity：GRK 5 Polyclonal Antibody detects endogenous levels of GRK 5 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：GRK5;GPRK5;G protein-coupled receptor kinase 5;G protein-coupled receptor kinase GRK5

Observed Band(KD)：68kD

Background： This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008],

Function：catalytic activity:ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.,function:Specifically phosphorylates the activated forms of G protein-coupled receptors.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,tissue specificity:Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.,

Subcellular Location：Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion.

Expression：Highest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.