Target：CDC20

Fields：Cell cycle;Oocyte meiosis;Ubiquitin mediated proteolysis;Human T-cell leukemia virus 1 infection;Viral carcinogenesis

Gene Name：CDC20

Protein Name：Cell division cycle protein 20 homolog

Human Gene Id：991

Human Swiss Prot No：Q12834

Mouse Gene Id：107995

Mouse Swiss Prot No：Q9JJ66

Rat Gene Id：64515

Rat Swiss Prot No：Q62623

Immunogen：The antiserum was produced against synthesized peptide derived from human p55CDC. AA range:81-130

Specificity：Cdc20 Polyclonal Antibody detects endogenous levels of Cdc20 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：CDC20;Cell division cycle protein 20 homolog;p55CDC

Observed Band(KD)：50kD

Background： CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008],

Function：developmental stage:Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.,function:Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated during mitosis, probably by maturation promoting factor (MPF).,PTM:Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint.,similarity:Belongs to the WD repeat CDC20/Fizzy family.,similarity:Contains 7 WD repeats.,subunit:Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1 (By similarity). Interacts with MAD2L

Subcellular Location：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .

Expression： Colon,Colon adenocarcinoma,Liver,Lymph,Muscle,Ovary,Skin,Spleen,Testis,